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Seed longevity

 
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PostPosted: Mon Jul 26, 2010 9:24 am    Post subject: Seed longevity Reply with quote

Hello there,

I would like to know how long may seeds of Ophrys and Orchis be conserved in the fridge, after their harvest and before they lose much of their viability? More simply put: what is the longevity of these seeds?

Many thanks,
WM.
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neil4768
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Joined: 23 Jan 2007
Posts: 83
Location: Sussex, England

PostPosted: Mon Jul 26, 2010 7:44 pm    Post subject: Reply with quote

If you dry them and keep them dry a long time, years, if not depending on the species a year is possible.
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SzPeter_hu
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Joined: 01 Jan 2008
Posts: 188
Location: Hungary

PostPosted: Mon Jul 26, 2010 8:52 pm    Post subject: Reply with quote

Ophrys seeds germinate after 6 months in nature, Orchis after about 1 year. So they remain viable for this length without refrigerating. If you keep them cool and dry, their life may be extended.

I used 7 year old seeds of Himantoglossum and Dactylorhiza with good results. I believe that most orchid seeds stay perfectly viable for at least 10 years (there are some with short viability but those are the exceptions). The loss of viability isn't a sudden change anyway. Therefore the exact life-time of seeds can't be determined. You can expect that as more and more years go by, less and less percent of the seeds will germinate. The Himantoglossum that I mentioned had about 100% viability after 7 years. Maybe this will decrease to 95% after 10 years, 80% after 15 years and so on. And who knows, maybe 1-2% will be viable even after a century.

Germination percentage of some seeds will increase with time. This means that 1 year old seeds may germinate better than fresh seeds because of the dormancy mechanism of the seeds. This also depends on the species.
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Wei Mi



Joined: 26 Jul 2010
Posts: 1
Location: France

PostPosted: Mon Jul 26, 2010 10:01 pm    Post subject: Reply with quote

Many thanks for your detailed answers.
SzPeter_hu, do you think the close-up observation (binocular microscope) of the embryo characteristics (color, form, turgescence) is an efficient indicator of chances of germination?

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Last edited by Wei Mi on Wed Jul 28, 2010 5:26 pm; edited 1 time in total
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SzPeter_hu
Seed Bank Manager


Joined: 01 Jan 2008
Posts: 188
Location: Hungary

PostPosted: Wed Jul 28, 2010 5:11 pm    Post subject: Reply with quote

Wei Mi wrote:

Do you think the close-up observation of the embryo characteristics is an efficient indicator of chances of germination?


No. Microscopic observation shows only whether the embryo is present or not. Some species are easy to germinate (Ophrys, Anacamptis, Dactylorhiza) while others are very difficult (Cypripedium, some Orchis). Anacamptis morio seeds with 5% embryos present may give you better germination than Orchis purpurea seeds with 100% embryos. Also the visual characteristics of the embryos do not show if the seeds are alive. I had bad experiences (with species that are very easy to propagate), when fresh seeds with fine embryos did not germinate at all. I don't know the reason why. Also embryos may loose viability after long time, or warm and humid storage but these changes are not visible. Fresh seeds may be viable but and after 50 years they are not. Still they appear the same under microscope.

A good close observation may predict which seeds are good and which are bad, but this is just 80% certainty. To be absolutely sure, a germination test must be done.
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SzPeter_hu
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Joined: 01 Jan 2008
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Location: Hungary

PostPosted: Wed Jul 28, 2010 6:54 pm    Post subject: Reply with quote

If you need further help, search for detailed information about the TTC method for testing seed viability.

As much as I know, it uses a chemical that is converted to a (photosensitive ?) red pigment in living cells. Any appearance of red colour indicates metabolism (i.e. live embryos) even before the first signs of swelling could be seen.
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neil4768
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Joined: 23 Jan 2007
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Location: Sussex, England

PostPosted: Wed Jul 28, 2010 8:59 pm    Post subject: Reply with quote

I was at Kew's Millennium Seed Bank at Wakehurst, and read an article about this process, but the seeds are unable to germinate after this.
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SzPeter_hu
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Joined: 01 Jan 2008
Posts: 188
Location: Hungary

PostPosted: Fri Jul 30, 2010 8:07 pm    Post subject: Reply with quote

Yes, this method actually wastes a sample to test viability. If you have a small quantity only, that may be a problem.
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