Terrestrial Orchid Forum

In-Vitro :: RE: Phaius Flava keiki? Is it possible to flask it?

Author: dllyon
Subject: Phais keiki
Posted: Tue Mar 09, 2010 3:41 pm (GMT 1)
Topic Replies: 4

Australian orchid growers frequently use pieces of terrestrial orchids to produce flasks for export and it should work fine with any type of growing plant material. I have tried it a couple of times and my experience has been that it is extremely difficult to get the plant material disinfected before flasking.
_________________
/s/ Danny Lyon

In-Vitro :: RE: Old medium?

Author: dllyon
Posted: Tue Mar 09, 2010 3:35 pm (GMT 1)
Topic Replies: 2

I have done this with seedlings that have particularly brittle or tangled roots and it seems to work just fine. There have been a few times when I am trying a new medium and it comes out a little soft that I have done this to give the seedlings a better support in the thin medium. It has worked very well in that situation also and apparently the additional water supports the growth longer before it becomes depleted.
_________________
/s/ Danny Lyon

Field trips :: RE: orchids.... Greece

Author: SzPeter_hu
Posted: Tue Mar 09, 2010 11:00 am (GMT 1)
Topic Replies: 12

Thanks for the identification Stephane. It was indeed beautiful to see thousands of these along the seashore.

Pleione :: RE: First Pleione in Bulgaria

Author: Hristo
Posted: Tue Mar 09, 2010 7:09 am (GMT 1)
Topic Replies: 10

Hi Lars, temperatures are fluctuating wildly this year, last 2 weeks we had day temps to +20c in the sun. Today is snowing, maybe 20cm on the ground and 2 days ago night temps were -10c! Keeping pleiones cool out of a fridge has been all but impossible so I have just let them do what they want. Not ideal, as I now have trays of pleiones on the dinning room floor because they can't go outside!!

The last -10c was unexpected and killed off a batch of unprotected Ophrys species in bulb frames, all in all this has been an 'annoying' winter. Last 2 years winter came, stayed for three months and went, this year it's been changing every 10 days!

Any of your Pleiones breaking dormancy?

In-Vitro :: RE: Old medium?

Author: Terra_Australis
Posted: Tue Mar 09, 2010 5:55 am (GMT 1)
Topic Replies: 2

I thought you were using liquid medium?

Cymbidium :: RE: Cymbidium sinense cv.He Zhi Hua�

Author: s1214215
Posted: Tue Mar 09, 2010 4:23 am (GMT 1)
Topic Replies: 5

Hi Dieter

Thanks. It seems to be very self-fertile too. All 8 flowers I pollinated have set pods.

I have a second plant and it looks 100% the same. Hopefully the seed can produce some variegated plants.

I have had no problems with any of the plants from the seller. They have all been good quality. I know some people take issue with buying from this seller due to some plants being wild sourced (which I dont want to get into again as it is such a complex issue). Many of the Cyms are clones though and the other cym clones I got last year are very good too. Sadly, I can not find pics of the peloric Cym ensifoliums I bought. I have one nice big pod on that from a selfing too.

Brett

In-Vitro :: Old medium?

Author: KY_plantguy
Subject: Old medium?
Posted: Mon Mar 08, 2010 11:35 pm (GMT 1)
Topic Replies: 2

It has been suggested that seedlings transplant/acclimatize (ex vitro) better if you do not remove the old medium because of less root disturbance. So I started to replate the whole mass of medium and seedlings on to new TC medium for some of seedlings. They seemed to respond better but if anyone has done this and if so, noticed any difference.

There are at least a few advantages. First, it would be quicker to replate. I don't have to pull seedlings off and put on a new medium. Because of the less root disturbance, roots of seedlings just continue to grow and penetrate the layer of the new medium.

One big problem is that the chance for contamination is a bit high especially the diameter of the old medium is fairly large, compared to the mouth of the new vessel. But seedlings are/should be fairly large at this point, so if that happens, I would just acclimatize them. Also, it doesn't look pretty sometimes, but it is less of my concern.

Any thought?

Pleione :: RE: First Pleione in Bulgaria

Author: larsb
Posted: Mon Mar 08, 2010 11:35 pm (GMT 1)
Topic Replies: 10

You must have your Pleione at much higher temperatures than i have. Mine is still hibernating.
_________________
Lars

In-Vitro :: RE: Ponerorchis medium

Author: KY_plantguy
Subject: Re: Ponerorchis medium
Posted: Mon Mar 08, 2010 11:23 pm (GMT 1)
Topic Replies: 10

goofy wrote:

from my own experience I cannot state they were growing that quickly.

my tubers were about 5 millimeters after first season.
then went dormant in the frige
and also after second season
the largest are not of flowering size

there was no difference in development
between sowing on oats agar or "organic medium"

I don't remember for sure what isolate they used, but I think it was from Liparis sp. The isolate seemed to have promoted better growth than the one isolated from Ponerorchis (and Cym. goeringii ?). I also think the author used the oat agar medium. So like I said, it all depends on fungal isolate, genotypes of species, etc.

Cymbidium :: RE: Cymbidium sinense cv.He Zhi Hua�

Author: goofy
Subject: Cymbidium
Posted: Mon Mar 08, 2010 9:16 pm (GMT 1)
Topic Replies: 5

hey Brett,
congrats.
seems to be a fantastic rare cultivar.
and looks really nice.
I like the "variegated".............

and quality looks far better
than the Cyps he offered last year

cheers
dieter
PS he just offers a nice book:
Field guide the orchids of china for 30 bucks incl postage to Europe
very interresting the "cheap" price
found other sources with much higher prices....

General Australian Orchids :: RE: some notes on germinating Pterostylis from seed

Author: andrew
Posted: Mon Mar 08, 2010 3:16 am (GMT 1)
Topic Replies: 14

fred wrote:

A different sequencing attempt was made by Christian (user Ahriman on Berthold's forum) but failed due to PCR issues.

The details of this little endeavor are detailed here (the second part of that thread is in English)

Thanks for the link, Fred. (I've only just figured out how to register with Berthold's forum and translate the text)

Field trips :: RE: orchids.... Greece

Author: stephane
Posted: Sat Mar 06, 2010 11:42 pm (GMT 1)
Topic Replies: 12

Hi
It is Pancratium maritimum. It is beautiful to see them, but it means also there is no more orchids to admire!!!!

Field trips :: RE: orchids.... Greece

Author: jamesmsss
Posted: Sat Mar 06, 2010 10:01 pm (GMT 1)
Topic Replies: 12

It may be an Ismene or Hymenocallis not sure which.

Field trips :: RE: orchids.... Greece

Author: SzPeter_hu
Posted: Sat Mar 06, 2010 7:42 pm (GMT 1)
Topic Replies: 12

At the end of last summer I've been on holiday on Santorini. The island was burned to death by the sun, so I could hardly find any plant that seemed to be alive. It was the most wonderful place I've ever seen, but I always had the terrible feeling that with every step I'm passing by hundreds of Ophrys tubers and I can't see them. Next time I will go there in spring.

By the way, I have seen large populations of a strange flower. It lived along the seashore on sandstone. Does anyone have a name for it? Here's a photo:

General Australian Orchids :: RE: some notes on germinating Pterostylis from seed

Author: fred
Posted: Sat Mar 06, 2010 11:57 am (GMT 1)
Topic Replies: 14

I updated the data on Tulasnella calospora with B1 trials and added a link on the forum main page.

General Australian Orchids :: RE: some notes on germinating Pterostylis from seed

Author: fred
Posted: Fri Mar 05, 2010 11:55 am (GMT 1)
Topic Replies: 14

andrew wrote:

Am I correct in thinking B1 is a type of Tulasnella?

B1 was isolated from a Dactylorhiza fuchsii in middle England by someone in the Hardy Orchid Society.

The true identity is still speculation, but everything points at Tulasnella (adpressed mucid growth). I sent out a sample for sequencing but the result was Ceratobasidium. It later became apparent that my B1 culture had two fungal strains growing in it - I was able to isolate woolly growth from "aerial hyphae" in a rich (20g/l OMA) medium from all my subcultures. This Ceratobasidium is not a germinator, btw.

A different sequencing attempt was made by Christian (user Ahriman on Berthold's forum) but failed due to PCR issues.

The details of this little endeavor are detailed here (the second part of that thread is in English)

General Australian Orchids :: RE: some notes on germinating Pterostylis from seed

Author: SzPeter_hu
Posted: Fri Mar 05, 2010 11:19 am (GMT 1)
Topic Replies: 14

neil4768 wrote:

you can get it from http://www.orchidsbypost.co.uk/ or http://www.johnsorchids.co.uk/
The email address are on the home pages, I know that both of them do sell it.

I got my B1 culture form www.johnsorchids.co.uk for free. Mr John Haggar was very helpful and he also gave me advice on how to use it.
I can also give you some of my cultures if you want, just send me a PM.

In-Vitro :: RE: Ponerorchis medium

Author: goofy
Subject: Re: Ponerorchis medium
Posted: Fri Mar 05, 2010 8:23 am (GMT 1)
Topic Replies: 10

KY_plantguy wrote:

If grown symbiotically, this species can produce blooming size tuber in 3~4 months. But you have to find the right kind, supposedly.

General Australian Orchids :: RE: some notes on germinating Pterostylis from seed

Author: Terra_Australis
Posted: Fri Mar 05, 2010 5:00 am (GMT 1)
Topic Replies: 14

I just noticed this thread is sitting under Mediterranean orchids... could we perhaps move it to the Australian section?

Cymbidium :: RE: Cymbidium sinense cv.He Zhi Hua�

Author: s1214215
Posted: Fri Mar 05, 2010 4:06 am (GMT 1)
Topic Replies: 5

Hi

Thanks for the correction. I worded the first entry poorly. I was told the plants of this cym where cloned in, and sourced from Taiwan. The variety did not originate there.

We rarely see variegated leaf cyms in Australia, so I hope I can get them to survive
customs there. They are a bit rough. Still that is some time away.

So far the flowers I pollinated look good and the seed pods are swelling quite well.

Brett

Field trips :: RE: orchids.... Greece

Author: stephane
Posted: Fri Mar 05, 2010 3:49 am (GMT 1)
Topic Replies: 12

Very nice pictures as usual and I am also amazed to see that you already saw species in bloom that I have seen in previous years only end of March, begining of April. I also understand very well your difficulty to put a tag on each plant you see as even with a field guide on orchids (like Delforge), it is not always easy to identify them. In which area are you?
To SzPeter: it is rather easy to find orchids in Crete. The worst enemy of orchids are sheeps and goats that will graze places until there is not much left. But still, it is rather easy to find plenty orchids, mainly in calcacerous places (Crete is also a paradise for geologists...)
S

General Australian Orchids :: RE: some notes on germinating Pterostylis from seed

Author: andrew
Posted: Fri Mar 05, 2010 3:34 am (GMT 1)
Topic Replies: 14

I'm very impressed that you were able to germinate any of them on B1. Well done! Maybe the problem with coccinea not germinating has less to do with it being a Diplodium and more to do with your other plants having nutans in the background. nutans is a very common species. I wouldn't be suprised if it's not very fussy about which fungus it uses for germination.

Just putting a thought out: Am I correct in thinking B1 is a type of Tulasnella? Given that Tulasnella calospora has been found to be associated with Diuris, has anyone tried germinating Diuris with B1?

Cymbidium :: RE: Cymbidium sinense cv.He Zhi Hua�

Author: KY_plantguy
Posted: Fri Mar 05, 2010 3:24 am (GMT 1)
Topic Replies: 5

Actually, '鶴之華' is a Cym. hoosai (sinense) cultivar from the main land that derived from either '金華山' or '金鳳錦' named in Nagasaki, Japan. Supposedly, it is named for sake of Nagasaki (鶴の港).

See:
http://blog.livedoor.jp/f0146/archives/2006-10.html?p=2
http://hallokosigaya.jugem.jp/
http://www.asahi-net.or.jp/~xv8m-oois/k-turu.htm

Field trips :: RE: orchids.... Greece

Author: KY_plantguy
Posted: Fri Mar 05, 2010 3:12 am (GMT 1)
Topic Replies: 12

Nice pics as usual

In-Vitro :: RE: Ponerorchis medium

Author: KY_plantguy
Subject: Re: Ponerorchis medium
Posted: Fri Mar 05, 2010 3:09 am (GMT 1)
Topic Replies: 10

fred wrote:

the "bad experience" quote comes from the reports I read online of people that tried symb/asymb IV, not publications. It appears that the germinated seeds often stop developing for some reason.

I have started my own batch, and just remember one thing. You are not supposed to culture/incubate at 25C. After stratification, you need to keep temperature between 15~20C for a few weeks, I think. I just finished 90 days of incubation in the fridge, and I put them in a colder lab.

If grown symbiotically, this species can produce blooming size tuber in 3~4 months. But you have to find the right kind, supposedly.